FC New York were founded in 2009, due to join the USL First Division. Due to the exodus of USL-1 clubs to join the North American Soccer League in 2010, FC New York invoked a clause allowing them to sit out the 2010 USL season. They made their debut in the USL Professional Division in the 2011 season, before playing in the National Premier Soccer League the following season. FC New York left the NPSL in December 2012 and folded soon after.
| Eastern Conference:|
Bethlehem Steel FC | Charleston Battery | Charlotte Independence | FC Cincinnati | Harrisburg City Islanders | Louisville City FC | New York Red Bulls II | Orlando City B | Ottawa Fury FC | Pittsburgh Riverhounds | Richmond Kickers | Rochester Rhinos | St. Louis FC | Tampa Bay Rowdies | Toronto FC II
Clubs on hiatus: